Negative staining and cryo-negative staining of macromolecules and viruses for TEM
نویسندگان
چکیده
منابع مشابه
Use of cryo-negative staining in tomographic reconstruction of biological objects: application to T4 bacteriophage.
Recent advances in electron microscopy and image analysis techniques have resulted in the development of tomography, which makes possible the study of structures neither accessible to X-ray crystallography nor nuclear magnetic resonance. However, the use of tomography to study biological structures, ranging from 100 to 500 nm, requires developments in sample preparation and image analysis. Inde...
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The first demonstration of intracytoplasmic virus by negative staining was that of Horne and Nagington (9) who subjected poliovirus-infected cells to freezing and thawing and negatively stained a suspension of the fragments. Dales (5) examined the attachment of some viruses to the cell surface by the negative staining of whole mounts of cells subjected to osmotic shock. Whittaker and Horne (15)...
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Malignant Mesothelioma, or mesothelioma, is a mesothelial-based malignancy that may occur in the pleura, pericardium and peritoneum. Mesothelioma is a very aggressive cancer with limited treatment, and a median survival of about 1 year. At times, the diagnosis of mesothelioma may be problematic. The final diagnosis of mesothelioma relies on histology and often is dependent upon immunohistochemi...
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The virus host-cell relationship has been studied in the electron microscope almost entirely by the thin sectioning technique. This method has given information about the sites of virus production within the cell and the appearance of virus particles at various stages in the growth cycle (1, 2). I t does not, however, permit high resolution studies on the substructure of individual particles or...
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BACKGROUND Negative-staining (NS), a rapid, simple and conventional technique of electron microscopy (EM), has been commonly used to initially study the morphology and structure of proteins for half a century. Certain NS protocols however can cause artifacts, especially for structurally flexible or lipid-related proteins, such as lipoproteins. Lipoproteins were often observed in the form of rou...
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ژورنال
عنوان ژورنال: Micron
سال: 2011
ISSN: 0968-4328
DOI: 10.1016/j.micron.2010.06.003